Quality ControlAQC™ Sperm Viability Quality Control
AQC107: Two human semen smears stained with Eosin-Nigrosin stain
When sperm aren’t moving, the question is are they dead or do they have a motility defect? The easiest method to differentiate between the two is a viability stain based on cell membrane exclusion. We recommend eosin staining with nigrosin counterstain. The extra nigrosin step takes a few seconds and makes the eosin staining much easier to detect. Live cells exclude the stain and appear white against the dark black-purple of the nigrosin. Live sperm are stained pink. Since this is a quantitative assay, it is high complexity and needs appropriate quality control. Document the QC quickly with Levey-Jennings plots included with the product.
The AQC™ Sperm Viability Quality Control complements the Sperm Viability Procedure and is an important part of the training exercises founs in The Andrology Trainer.
Specifications
- Shelf Life: Smears are stable for 6 months from date of shipment. Store in a dark dry environment at room temperature.
- FDA Clearance: Class I, For In Vitro Diagnosis
- Customization Available: Number of smears
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